California NanoSystems Institute
CNSI
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Dino Di Carlo, Ph.D.

   
Associate Professor, Bioengineering
Member, California NanoSystems Institute

Education:
Degrees:
Ph.D., University of California, Berkeley and San Francisco, 2006
B.S., University of California Berkeley, 2002

Contact Information:
Email Address: dicarlo@seas.ucla.edu
Work Email Address: dicarlo@seas.ucla.edu
Office Address: UCLA
Los Angeles, CA 90095
UNITED STATES
Home Page: http://dicarlo.bol.ucla.edu/
Work Phone Number: 310-983-3235
Technical Research Interest:

We are exploiting unique physics, microenvironment control, and the potential for automation associated with miniaturized systems for applications in basic biology, medical diagnostics, and cellular engineering. Current research is focused on: (i) Quantitative cell biology and mechanics of cancer metastasis. Microfluidic methods to control the surface chemistry, mechanical, and soluble environment are well suited to address questions associated with cell migration and movement. We are particularly interested in the process of cancer metastasis and intravasation. (ii) Nonlinear microfluidics. Nonlinear fluid dynamic effects are usually not considered in microfluidic systems but may provide simple methods to manipulate and sort rare populations of cells at high-throughputs. We are studying the physical basis of inertial migration of particles and engineering novel portable and robust diagnostic and analysis systems using this phenomenon for applications in the developed and developing world. (iii) Microfluidic directed cellular evolution. Microfluidic technologies may offer advantages in creating new useful selection criteria for cellular evolution. Besides gaining an understanding of dominant molecular pathways in controlling these behaviors, the resultant evolved cell populations and genetic modifications may be useful for therapeutic applications.



Selected Publications:

Hamed Amini, Elodie Sollier, Westbrook M. Weaver, and Dino Di Carlo, Intrinsic particle-induced lateral transport in microchannels, PNAS, 2012, 1-6.
Keisuke Goda, Ali Ayazi, Daniel R. Gossett, Jagannath Sadasivama, Cejo K. Lonappan, Elodie Sollier, Ali M. Fard, Soojung Claire Hur, Jost Adam, Coleman Murray, Chao Wang, Nora Brackbill, Dino Di Carlo, and Bahram Jalali , High-throughput single-microparticle imaging flow analyzer, PNAS, 2012, 1-6.
Daniel R. Gossetta, Henry T. K. Tse, Serena A. Lee, Yong Ying, Anne G. Lindgren, Otto O. Yang, Jianyu Rao, Amander T. Clark, and Dino Di Carlo, Hydrodynamic stretching of single cells for large population mechanical phenotyping, PNAS, 2012, 109 (20), 7630-7635.
Henry Tat Kwong Tse, Westbrook McConnell Weaver, Dino Di Carlo, Increased Asymmetric and Multi-Daughter Cell Division in Mechanically Confined Microenvironments, PLoS ONE, 2012, 7 (6), 1-8.
Hur SC, Tse HTK, Di Carlo D., Sheathless inertial cell ordering for extreme throughput flow cytometry, Lab on a Chip , 2010, 10 (274), 274-280.
Russom A, Gupta AK, Nagrath S, Di Carlo D, Edd JF, Toner M. , Differential Inertial Focusing of Particles in Curved Low-Aspect-Ratio Microchannels, New Journal of Physics, 2009, 11, 075025.
Di Carlo D., Inertial Microfluidics Review Article, Lab on a Chip , 2009, 9, 3038-3046.
Gossett DR, Di Carlo D., Particle Focusing Mechanisms in Curving Confined Flows, Analytical Chemistry, 2009, 81 (20), 8459-8465.
Di Carlo D, Edd JF, Humphry KJ, Stone HA, Toner M., Particle segregation and dynamics in confined flows, Physical Review Letters, 2009, 102 (9), 094503.
Tseng P, Di Carlo D, Judy JW., Rapid and Dynamic Intracellular Patterning of Cell-Internalized Magnetic Fluorescent Nanoparticles, Nano Letters, 2009, 9 (8), 3053–3059.
Chia-Hsien, Hsu Di Carlo, D. Chen, C. Irimia, D. Toner, M., Microvortex for focusing, guiding and sorting of particles, Lab on a Chip, 2008, 8 (12), 2128-2134.
Edd, J. F. Di Carlo, D. Humphry, K. J. Koster, S. Irimia, D. Weitz, D. A. Toner, M., Controlled encapsulation of single-cells into monodisperse picolitre drops, Lab on a Chip, 2008, 8 (8), 1262-1264.
Di Carlo, D. Edd, J. F. Irimia, D. Tompkins, R. G. Toner, M., Equilibrium separation and filtration of particles using differential inertial focusing, Analytical Chemistry, 2008, 80 (6), 2204-2211.
Wu LY, Di Carlo D, Lee LP., Microfluidic Self-Assembly of Tumor Spheroids for Anti-Cancer Drug Discovery, Biomedical Microdevices , 2008, 2, 197-202.
Di Carlo, D. Irimia, D. Tompkins, R. G. Toner, M., Continuous inertial focusing, ordering, and separation of particles in microchannels, Proceedings of the National Academy of Sciences of the United States of America, 2007, 104 (48), 18892-18897.
Di Carlo, D. Wu, L. Y. Lee, L. P., Dynamic single cell culture array, Lab on a Chip, 2006, 6 (11), 1445-1449.
Di Carlo, D. Lee, L. P., Dynamic single-cell analysis for quantitative biology, Analytical Chemistry, 2006, 78 (23), 7918-7925.
Ionescu-Zanetti, C. Nevill, J. T. Di Carlo, D. Jeong, K. H. Lee, L. P., Nanogap capacitors: Sensitivity to sample permittivity changes, Journal of Applied Physics, 2006, 99 (2), 5.
Di Carlo, D. Aghdam, N. Lee, L. P., Single-cell enzyme concentrations, kinetics, and inhibition analysis using high-density hydrodynamic cell isolation arrays, Analytical Chemistry, 2006, 78 (14), 4925-4930.
Ionescu-Zanetti, C. Wang, L. P. Di Carlo, D. Hung, P. Di Blas, A. Hughey, R. Lee, L. P., Alkaline hemolysis fragility is dependent on cell shape: Results from a morphology tracker, Cytometry Part A, 2005, 65A (2), 116-123.
Di Carlo, D. Ionescu-Zanetti, C. Zhang, Y. Hung, P. Lee, L. P., On-chip cell lysis by local hydroxide generation, Lab on a Chip, 2005, 5 (2), 171-178.
Di Carlo, D. Jeong, K. H. Lee, L. P., Reagentless mechanical cell lysis by nanoscale barbs in microchannels for sample preparation, Lab on a Chip, 2003, 3 (4), 287-291.