California NanoSystems Institute
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Laurent Bentolila, Ph.D.

Scientific Director, Advanced Light Microscopy/Spectroscopy Lab
Scientific Director, Macro-Scale Imaging Lab
Researcher, California NanoSystems Institute

Ph.D., Pasteur Institute, Paris and Paris-XI University, Orsay, France, 1998
M.S., Paris-XI University, Orsay, France, 1992
B.S., Pari-XI University, Orsay, France, 1991

Contact Information:
Work Email Address:
Work Address: Advanced Light Microscopy/Spectroscopy Laboratory
California NanoSystems Institute
570 Westwood Plaza
Bldg 114
Los Angeles, CA 90095
Office Phone Number: (310) 983-1076
Fax Number: (310) 267-4918
Additional Information:

Dr. Laurent A. Bentolila is a biochemist and a molecular geneticist who joined the Department of Chemistry and Biochemistry in 2002. He currently serves as the Scientific Director of both the Advanced Light Microscopy/Spectroscopy Laboratory and the Macro-Scale Imaging Laboratory at CNSI. Dr. Bentolila earned his B.S. in Biochemistry and M.S. in Genetics from Paris-XI University, Orsay and Ph.D. in Molecular Genetics and Immunology from the Pasteur Institute, Paris. He was a European Molecular Biology Organization Postdoctoral fellow at the University of California, Berkeley where he worked on the recombination and expression of antigen receptor genes in lymphocytes. Dr. Bentolila is an elected member of the CNSI Executive Committee and Education Committee. He is actively teaching in Biological Microscopy (MIMG 105) and Introduction to Molecular Imaging (M248). Since becoming Scientific Director of the Advanced Light Microscopy/Spectroscopy Laboratory at CNSI, Dr. Bentolila has developed and assembled a unique collection of custom-made and commercial light microscopy tools for the application of novel spectroscopic methods and advanced microscopy techniques used for the study of macromolecules, cellular dynamics and nano-scale characterization of biomaterials.

Selected Publications:

Wang H, Gorocs Z, Luo W, Zhang Y, Rivenson Y, Bentolila LA and Ozcan A, Computational out-of-focus imaging increases the space-bandwidth product in lens-based coherent microscopy, Optica, 2016, in press.
Reinig MR, Novack SW, Tao X, Ermini F, Bentolila LA, Roberts DG, MacKenzie-Graham A, Godshalk SE, Raven MA and Kubby J, Adaptive optics microscopy enhances image quality in deep layers of CLARITY processed brains of YFP-H mice, Proc. SPIE 9690, 2016, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation (969008).
Bentolila LA, Prakash R, Mihic-Probst D, Wadehra M, Kleinman HK, Carmichael TS, Péault B, Barnhill RL and Lugassy C. , Imaging of Angiotropism/Vascular Co-Option in a Murine Model of Brain Melanoma. Implications for Melanoma Progression along Extravascular Pathways, Scientific Reports, 2016, 6, 23834.
Sonn GA, Behesnilian AS, Jiang ZK, Zettlitz KA, Lepin EJ, Bentolila LA, Knowles SM, Lawrence DJ, Wu AM, Reiter RE., Fluorescent Image-Guided Surgery with an Anti-Prostate Stem Cell Antigen (PSCA) Diabody Enables Targeted Resection of Mouse Prostate Cancer Xenografts in Real-Time, Clin. Cancer Res, 2015, [Epub ahead of print].
Bentolila LA, Photoluminescent quantum dots in imaging, diagnostics and therapy, In: Applications of Nanoscience in Photomedicine, Chap 5, (Hamblin MR and Avci P. Eds) Elsevier Woodhead Publishing Series in Biomedicine, Number 24, Cambridge, United Kingdom, 2015, 24 (49), 77-104.
Chen AL, Kim EW, Toh JY, Vashisht AA, Rashoff AQ, Van C, Huang AS, Moon AS, Bell HN, Bentolila LA, Wohlschlegel JA, Bradley PJ., Novel components of the Toxoplasma inner membrane complex revealed by BioID, MBio, 2015, 6 (1), e02357-14.
Wei Q, Qi H, Luo W, Tseng D, Bentolila LA, Wu TT, Sun R and Ozcan A, Single Nanoparticle and Virus Detection Using a Smart Phone Based Fluorescence Microscope, Conference on Lasers and Electro-Optics (CLEO), 2014 (AW3L.1).
Lugassy C, Zadran S, Bentolila LA, Wadehra M, Prakash R, Carmichael TS, Kleinman H, Péault B, Larue L and Barnhill RL, Angiotropism, pericytic mimicry and extravascular migratory metastasis in melanoma: an alternative to Intravascular Cancer Dissemination, Cancer Microenvironment, 2014, 7 (3), 139-52.
Kisalu NK, Langousis GD, Bentolila LA, Ralston KS, Hill KL, Mouse infection and pathogenesis by Trypanosoma brucei motility mutants, Cell Microbiol, 2014, 16 ((6)), 912-924.
Mittal N, Roberts K, Pal K, Bentolila LA, Fultz E, Minasyan A, Cahill C, Pradhan A, Conner D, Defea K, Evans C, Walwyn W, Select G-Protein-Coupled Receptors Modulate Agonist-Induced Signaling via a ROCK, LIMK, and β-Arrestin 1 Pathway, Cell Report, 2013, 5 (4), 1010-1021..
Wei Q, Qi H, Luo W, Tseng D, Jung Ki S, Wan Z, Göröcs Z, Bentolila LA, Wu T, Sun R, and Ozcan A, Fluorescent Imaging of Single Nanoparticles and Viruses on a Smart-Phone, ACS Nano, 2013, 7 (10), 9147-9155.
Wend P, Runke S, Wend K, Anchondo B, Yesayan M, Jardon M, Hardie N, Loddenkemper C, Ulasov I, Lesniak MS, Wolsky R, Bentolila LA, Grant SG, Elashoof D, Lehr S, Latimer JJ, Bose S, Sattar H, Krum SA, Miranda-Carboni GA, WNT10B/β-catenin Signaling induces HMGA2 and proliferation in metastatic triple-negative breast cancer, EMBO Mol. Med, 2013, 5 (2), 264-279.
Mitchell-Jordan S, Chen H, Franklin S, Stefani E, Bentolila LA, Vondriska TM, Features of endogenous cardiomyocyte chromatin revealed by super-resolution STED microscopy, J Mol Cell Cardiol, 2012, 53 (4), 552-8.
Sharma S, Santiskulvong C, Bentolila LA, Rao J, Dorigo O, Gimzewski JK., Correlative nanomechanical profiling with super-resolution F-actin imaging reveals novel insights into mechanisms of cisplatin resistance in ovarian cancer cells, Nanomedicine, 2012, 8 (5), 757-766.
Gu Z, Zhao M, Sheng Y, Bentolila LA and Tang Y, Detection of Mercury Ion by Infrared Fluorescent Protein and Its Hydrogel-Based Paper Assay, Anal. Chem, 2011, 83 (6), 2324-2329.
Bentolila LA, Direct in situ hybridization with oligonucleotide functionalized quantum dot probes, Methods Mol. Biol, 2010, 659, 147-163.
Ebenstein Y and Bentolila LA, Single-molecule detection: Focusing on the objective, Nature Nanotechnology, 2010, 5 (2), 99-100.
Coffinier C, Jung HJ, Li Z, Nobumori C, Yun UJ, Farber EA, Davies BS, Weinstein MM, Yang SH, Lammerding J, Farahani JN, Bentolila LA, Fong LG and Young SG, Direct synthesis of lamin A, bypassing prelamin A processing, causes misshapen nuclei in fibroblasts but no detectable pathology in mice, J. Biol. Chem, 2010, 285 (27), 20818-20826.
Yang J, Kickhoefer VA, Ng BC, Gopal A, Bentolila, LA, John S, Tolbert SH and Rome LH, Vaults are dynamically unconstrained cytoplasmic nanoparticles capable of rapid half subunit exchange in vivo, ACS Nano, 2010, 4 (12), 7229-7240.
Bentolila L.A., Ebenstein Y. and Weiss S., Quantum dots for in vivo small animal imaging, Journal of Nuclear Medicine, 2009, 50 (4), 493-496.
Schipper ML, Iyer G, Koh AL, Cheng Z, Ebenstein Y, Aharoni A, Keren S, Bentolila LA, Li J, Rao J, Chen X, Banin U, Wu AM, Sinclair R, Weiss S and Gambhir SS, Particle size, surface coating, and PEGylation influence the biodistribution of quantum dots in living mice, Small, 2009, 5 (1), 126-134.
Bentolila L.A., Doose S., Ebenstein Y., Iyer G., Li J.J., Michalet X., Pinaud F., Tsay J. and Weiss S., Peptide-functionalized quantum dots for live diagnostic imaging and therapeutic applications, In: Inorganic Nanoprobes for Biological Sensing and Imaging, 2009, Chap. 3 (Mattoussi H. and Cheon J., Eds), Artech House Publisher, Boston, Massachusetts), 45-69.
Michalet X., Bentolila L.A. and Weiss S. , Molecular imaging: physics and bioapplications of quantum dots, Advances in Medical Physics, 2008, Vol. II (Wolbarst A.B., Mossman K.L. and Hendee W.R., Eds), 111-127.
Bentolila L.A., Michalet X. and Weiss S., Quantum optics: colloidal fluorescent semiconductor nanocrystals (quantum dots) in single-molecule detection and imaging, In: Single-Molecules and Biotechnology, 2008, Chap. 3 ((Rigler R. and Vogel E., Eds), Springer-Verlag, Berlin Heidelberg), 53-81.
Schipper M.L., Cheng Z., Lee S.-W., Bentolila L.A., Iyer G., Rao J., Chen X., Wu A.M., Weiss S. and Gambhir S.S., MicroPET-based biodistribution of quantum dots in living mice, Journal of Nuclear Medicine, 2007, 48 (9), 1511-1518.
Michalet X., Bentolila L.A. and Weiss S., Molecular imaging, In: Advances in Medical Physics, 2006, Vol. I (Chap. 7 (Wolbarst A.B., Zamenhof R.G. and Hendee W.R., Eds), Medical Physics Publishing, Madison, Wisconsin), 181-200.
Bentolila L.A. and Weiss S., Single-step multicolor fluorescence In situ hybridization using semiconductor quantum dot-DNA conjugates , Cell Biochemistry and Biophysics, 2006, 45 (1), 59-70.
Pinaud F., Michalet X., Bentolila L.A., Tsay J.M., Doose S., Li J.J., Iyer G. and Weiss S., Advances in fluorescence imaging with quantum dot bio-probes, Biomaterials, 2006, 27 (9), 1679-1687.
Iyer G., Pinaud F., Tsay J., Li J.J., Bentolila L.A., Michalet X. and Weiss S., Peptide coated quantum dots for biological applications, IEEE Transactions on Nanobioscience, 2006, 5 (4), 231-238.
Wang L., Radu C.G., Yang L.V., Bentolila L.A., Riedinger M. and Witte O.N., Lysophosphatidylcholine-induced Surface Redistribution Regulates Signaling of the Murine G Protein-coupled Receptor G2A, Molecular Biology of the Cell, 2005, 16 (5), 2234-2247.
Michalet X., Pinaud F.F., Bentolila L.A., Tsay J.M., Doose S., Li J.J., Sundaresan G., Wu A.M., Gambhir S.S. and Weiss S., Quantum dots for live cells, in vivo imaging, and diagnostics, Science, 2005, 307 (5709), 538-544.
Bentolila L.A., Michalet X., Pinaud F.F., Tsay J.M., Doose S., Li J.J., Sundaresan G., Wu A.M., Gambhir S.S. and Weiss S., Quantum dots for molecular imaging and cancer medicine, Discovery Medicine, 2005, 5 (26), 213-218.
Michalet X., Pinaud F.F., Bentolila L.A., Tsay J.M., Doose S., Li J.J., Iyer G. and Weiss S., Peptide-coated semiconductor nanocrystals for biomedical applications, Proceedings of the SPIE - The International Society for Optical Engineering, 2005, 5704 (1), 57-68.
Tsay J.M., Pflughoefft M., Bentolila L.A. and Weiss S., Hybrid approach to the synthesis of highly luminescent CdTe/ZnS and CdHgTe/ZnS nanocrystals, Journal of the American Chemical Society , 2004, 126 (7), 1926-1927.
Bentolila L.A. and Weiss S., Biological quantum dots go live, Physics World, 2003, 16, 23-24.
Bentolila L.A., Olson S., Marshall A., Rougeon F., Paige C.J., Doyen N. and Wu G.E., Extensive junctional diversity in Ig light chain genes from early B cell progenitors of mu MT mice, Journal of Immunology, 1999, 162 (4), 2123-2128.
Bentolila L.A., Study of two isoforms of Terminal Deoxynucleotidyl Tranferase (TdT) in mouse. Modification of the expression of the major form in a mouse transgenic model, Doctor of Philosophy Thesis, 1998, The Pasteur Institute and Paris-XI University, 1-162.
Marshall A.J., Doyen N., Bentolila L.A., Paige C.J. and Wu G.E., Expression of TdT during neonatal life alters DH reading frame usage and Ig receptor-dependent selection of V regions, Journal of Immunology, 1998, 161 (12), 6657-6663.
Bentolila L.A., Wu G.E., Nourrit F., Fanton d'Andon M., Rougeon F. and Doyen N., Constitutive expression of terminal deoxynucleotidyl transferase in transgenic mice is sufficient for N region diversity to occur at any Ig locus throughout B cell differentiation, Journal of Immunology, 1997, 158 (2), 715-723.
Connor A., Fanning L., Bentolila L.A. and Wu G.E., RSS plasmid substrate assays for V(D)J rearrangement, In: Immunol. Methods Manual, 1997, Chap. 4.3 ((Lefkovits, I., Ed.) London Academic Press), 260-268.
Bentolila L.A., Fanton d'Andon M., Nguyen Q.T., Martinez O., Rougeon F. and Doyen N., The two isoforms of mouse terminal deoxynucleotidyl transferase differ in both the ability to add N regions and subcellular localization, The EMBO Journal, 1995, 14 (17), 4221-4229.
Doyen N., d'Andon M.F., Bentolila L.A., Nguyen Q.T. and Rougeon F., Differential splicing in mouse thymus generates two forms of terminal deoxynucleotidyl transferase, Nucleic Acids Research, 1993, 21 (5), 1187-1191.