California NanoSystems Institute
CNSI
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Harold Monbouquette, Ph.D.

   
Professor, Chemical and Biomolecular Engineering
Member, California NanoSystems Institute, Neuroengineering Training Program

Education:
Degrees:
Ph.D., North Carolina State University, 1987
M.S., University of California, Davis, 1984
B.S., University of Massachusetts, Amherst, 1982
A.B., Harvard College, 1979

Professional Societies:
American Institute of Chemical Engineers
American Chemical Society

Contact Information:
Email Address: hmonbouq@ucla.edu
Work Email Address: harold@seas.ucla.edu
Work Address: UCLA
Department of Chemical Engineering
Box 951592
Los Angeles, CA 90095
UNITED STATES
ARR Papers: http://www.cnsi.ucla.edu/arr/personnel-papers?personnel_id=114321
Home Page: http://www.chemeng.ucla.edu/HMonbouquette/index.html
Fax Number: (310) 206-4107
Work Phone Number: (310) 825-8946
Research Interests:

Molecular engineering of innovative systems that mimic the biological (i.e., biomimetic systems) in efforts to solve technological problems. Future advances in many areas of interest to chemical engineers including catalysis, advanced materials, and separations will require engineering at the molecular scale. Since the direct manipulation of individual molecules presents obvious technological difficulties, much research has focused on self-assembling systems. The living cell is the ultimate self-assembled system with unrivaled capabilities in separations, sensing and synthesis; and lipid bilayer membranes are critical self-assembled structures for cell functioning as they serve, for example, to define cell boundaries, to compartmentalize reactions in controlled environments, and to provide scaffolding for the molecular machines involved in signal and energy transduction. My research projects on vesicular biomimetic membranes for separations, metal ion detection and nanoparticle synthesis; and on lipid-modified electrodes for biosensor development and for electroenzymatic chiral synthesis were conceived with the attributes of cell membranes in mind. Another common theme in my research, therefore, is the reliance on self-assembly to produce new technology that is critically dependent on molecular-scale structure.


Technical Research Interest:

The Monbouquette group conducts research on biosensors, the biotechnological applications of extremely thermophilic microorganisms, protein nanocapsule technology, and on the molecular engineering of surfaces. The group is collaborating with a UCLA neuroscientist on the micromachining of biosensors for in vivo monitoring of neurotransmitter release and uptake. In other work, a reporter enzyme has been engineered to behave as a molecular switch for several sensing applications under exploration including the detection of environmental toxins and the high-throughput screening of drug candidates. Extremely thermophilic microbes are being investigated as a source of enzymes and metabolic pathways useful in specialty chemical synthesis and of highly stable lipids for potential applications in drug delivery and in biosensor design. The Monbouquette laboratory also is discovering conditions for the controlled opening and closing of naturally occurring protein nanocapsules, in collaboration with Prof. Rome's group, such that they may be useful in drug delivery or in the synthesis of nanomaterials. Finally, the group is pursuing development of a process for the creation of complex, userdefined surface patterns with ~2-3 nm feature size. This new nanopatterning concept is based on the use of electrophoretically mobile, photocatalytic nanoparticles as "pens" to draw nanopatterns on a photocatalytically reactive surface.



Selected Publications:

Chiu ML, Lai D, Monbouquette HG, An influenza hemagglutinin A peptide assay based on the enzyme-multiplied immunoassay technique, J Immunoassay Immunochem, 2011, 32 (1), 1-17.
Radhakrishnan C, Lo MK, Knobler CM, Garcia-Garibay MA, Monbouquette HG, Capping-Ligand Effect on the Stability of CdSe Quantum Dot Langmuir Monolayers, Langmuir, 2011, Epub ahead of print.
Galatsis K, Wang KL, Ozkan M, Ozkan CS, Huang Y, Chang JP, Monbouquette HG, Chen Y, Nealey P, Botros Y, Patterning and templating for nanoelectronics, Adv Mater, 2010, 22 (6), 769-78.
Lim S, Springstead JR, Yu M, Bartkowski W, Schröder I, Monbouquette HG, Characterization of a key trifunctional enzyme for aromatic amino acid biosynthesis in Archaeoglobus fulgidus, Extremophiles, 2009, 13 (1), 191-8.
Lai D, Lluncor B, Schröder I, Gunsalus RP, Liao JC, Monbouquette HG, Reconstruction of the archaeal isoprenoid ether lipid biosynthesis pathway in Escherichia coli through digeranylgeranylglyceryl phosphate, Metab Eng, 2009, 11 (3), 184-91.
Lisa E. Goldsmith, Melody Pupols, Valerie A. Kickhoefer, Leonard H. Rome and Harold G. Monbouquette, Utilization of a Protein “Shuttle” To Load Vault Nanocapsules with Gold Probes and Proteins, ACS Nano, 2009, 3 (10), 3175–3183.
Lai, D. Springstead, J. R. Monbouquette, H. G., Effect of growth temperature on ether lipid biochemistry in Archaeoglobus fulgidus, Extremophiles, 2008, 12 (2), 271-8.
Benny C. Ng, Marcella Yu, Ajaykumar Gopal, Leonard H. Rome, Harold G. Monbouquette and Sarah H. Tolbert, Encapsulation of Semiconducting Polymers in Vault Protein Cages, Nano Lett, 2008, 8 (10), 3503–3509.
Hsiang-Yu Chen, Michael K. F. Lo, Guanwen Yang, Harold G. Monbouquette & Yang Yang, Nanoparticle-assisted high photoconductive gain in composites of polymer and fullerene, Nature Nanotechnology, 2008, 3 (9), 543 - 7 .
Yu, M. Ng, B. C. Rome, L. H. Tolbert, S. H. Monbouquette, H. G., Reversible pH lability of cross-linked vault nanocapsules, Nano Lett, 2008, 8 (10), 3510-5.
Wassum KM, Tolosa VM, Wang J, Walker E, Monbouquette HG, Maidment NT, Silicon Wafer-Based Platinum Microelectrode Array Biosensor for Near Real-Time Measurement of Glutamate in Vivo, Sensors Basel Sensors, 2008, 8 (8), 5023-36.
Walker E, Wang J, Hamdi N, Monbouquette HG, Maidment NT, Selective detection of extracellular glutamate in brain tissue using microelectrode arrays coated with over-oxidized polypyrrole, Analyst, 2007, 132 (11), 1107-11.
Goldsmith, L. E. Yu, M. Rome, L. H. Monbouquette, H. G., Vault nanocapsule dissociation into halves triggered at low pH, Biochemistry, 2007, 46 (10), 2865-75.
N. Hamdi, J. Wang, E. Walker, N.T. Maidment, H.G. Monbouquette, An electroenzymatic L-glutamate microbiosensor selective against dopamine, J. Electroanal. Chem, 2006, 591 (1), 33-40.
Radhakrishnan, C. Lo, M. K. Warrier, M. V. Garcia-Garibay, M. A. Monbouquette, H. G., Photocatalytic reduction of an azide-terminated self-assembled monolayer using CdS quantum dots, Langmuir, 2006, 22 (11), 5018-24.
F.H. Ko and H.G. Monbouquette, Photometric and electrochemical enzyme-multiplied assay techniques using beta-galactosidase as reporter enzyme, Biotechnol. Progr, 2006, 22 (3), 860-865.
J. Wang, N.V. Myung, M. Yun and H.G. Monbouquette, Glucose oxidase entrapped in polypyrrole on high-surface-area Pt electrodes: a model platform for sensitive electroenzymatic biosensors, Journal of Electroanalytical Chemistry, 2005, 575 (1), 139-146.
N. Hamdi, J. Wang, H.G. Monbouquette, Polymer Films as Permselective Coatings for H2O2-Sensing Electrodes, J. Electroanal. Chem, 2005, 581 (2), 258-264.
I. Schroeder, A. Vadas, E. Johnson, S. Lim and H.G. Monbouquette, A novel archaeal alanine dehydrogenase homologous to ornithine cyclodeaminase and mu-crystallin, Journal of Bacteriology, 2004, 186 (22), 7680-7689.
Lim, S. Schroder, I. Monbouquette, H. G., A thermostable shikimate 5-dehydrogenase from the archaeon Archaeoglobus fulgidus, FEMS Microbiol Lett, 2004, 238 (1), 101-6.
Warrier, M; Lo, MK; Monbouquette, H; Garcia-Garibay, MA, Photocatalytic reduction of aromatic azides to amines using CdS and CdSe nanoparticles, Photochem Photobiol Sci, 2004, 3 (9), 859-63.
D.T. Gallagher, H.G. Monbouquette, I. Schroeder, H. Robinson, M.J. Holden and N.N. Smith, Structure of Alanine Dehydrogenase from Archaeoglobus: Active Site Analysis and Relation to Bacterial Cyclodeaminases and Mammalian mu Crystallin, Journal of Molecular Biology, 2004, 342 (1), 119-130.
Wang, J. C. Aucoin-Barry, D. Manuelian, D. Monbouquette, R. Reisman, M. Gray, W. Block, P. C. Block, E. H. Ladenheim, M. Simon, D. I. , Incidence of aspirin nonresponsiveness using the Ultegra Rapid Platelet Function Assay-ASA, Am J Cardiol, 2003, 92 (12), 1492-4.
Vadas, A. J. Schroder, I. Monbouquette, H. G. , Room-temperature synthesis of L-alanine using the alanine dehydrogenase of the hyperthermophilic archaeon Archaeoglobus fulgidus, Biotechnol Prog, 2002, 18 (4), 909-11.